This is Italian. Ezio is Italian. At first, she spoke Italian. She interacted with Lucy in Modern Line. She explained to Desmond that animus automatically translated into English because Desmond didn't know Italian. There is still a small amount of commonly used Italian. For example, Va bene (ok), Requiesca (rest in peace) and Grazie (thank you) in pace. Signore (sir/madam) said goodbye to her sister (because I opened the subtitles, I can see these words and sentences. )