At present, nasopharyngeal swab, oropharyngeal swab and sputum are commonly used in nucleic acid detection, each of which has its own advantages and disadvantages. The pharynx, located in the deep part of the nose and mouth, is divided into three parts: nasopharynx, oropharynx and laryngopharynx from outside to inside. Nasopharyngeal swab collection is what we often call "nose poking". It is to insert the sampling tool into the nasal cavity to collect nasopharyngeal specimens. The detection rate and accuracy will be higher, but the operation is more complicated and more difficult. Some people are not easy to adapt and their acceptance is slightly lower.