After optimizing the wild soft-shelled turtle, Martin Science and Technology Company developed the high-tech and high-quality breeding technology of soft-shelled turtle. Firstly, the wild Trionyx sinensis, the preferred tetrad parent, was used for self-propagation, and then the wild hybrid parent Trionyx sinensis was cultivated. Then the wild hybrid parent Trionyx sinensis was mated with each other to breed the unrelated Huang Yun brand Trionyx sinensis with the same gene and new parents, thus inventing the breeding technology of "425- 1-30" high-quality Trionyx sinensis. The technical cycle of improved breed of Trionyx sinensis is 30 years, and each cycle has four breeding periods and two breeding periods, in which the breeding period of parent Trionyx sinensis is 5 years and the breeding period is 65,438+00 years, and four varieties indirectly related to parent Trionyx sinensis are obtained in each breeding period. The breeding technology of improved varieties of Trionyx sinensis not only optimizes the Yellow River strain of Trionyx sinensis, but also avoids the defects caused by inbreeding of Trionyx sinensis, such as deformity, variety degradation, poor quality, low immunity, low survival rate and slow growth, which ensures the excellent, stable and safe quality of Trionyx sinensis and provides technical support for the protection and breeding of high-quality original and improved Yellow River strains of Trionyx sinensis.
In order to ensure the quality, stability and safety of the Yellow River strain of Trionyx sinensis, they established a scientific germplasm identification method to ensure that the "Huang Yun" brand Trionyx sinensis cultivated by the breeding technology of "425- 1-30" is an excellent variety. The method of combining morphological measurement and structural observation is used to select seeds; Biochemical analysis by isozyme method confirmed that the biochemical genetic characteristics and genetic structure of the cultured "Huang Yun" brand Trionyx sinensis kept the safety and stability of the original germplasm. Using polymerase chain reaction (PCR) and restriction length polymorphism (RELP) techniques, the cytochrome (cyt) gene of mitochondrial DNA(mtDNA) of several populations of Trionyx sinensis was analyzed. It was concluded that the genome of "Huang Yun" brand Trionyx sinensis was obviously out of proportion to other populations, with high genetic diversity and fast growth. The protein of muscle tissue specificity of Trionyx sinensis was analyzed by two-dimensional electrophoresis, and it was found that the protein of "Huang Yun" brand Trionyx sinensis was significantly higher than that of other Trionyx sinensis, indicating that its genetic diversity was preserved.
Compared with the original breeding of wild Trionyx sinensis in 2000-2003, the improved Huang Yun brand Trionyx sinensis bred by the high-quality breeding technology of "425- 1-30" shows that the Huang Yun brand Trionyx sinensis has the advantages of big body, thin body, thick skirt, yellow or yellowish-brown back armor, yellowish belly armor, strong physique and flexible activities.
In 2005, the wild original species of Trionyx sinensis and the bred "Huang Yun" high-quality fry of Trionyx sinensis were approved as original species and improved species by Shandong Provincial Aquatic Original Species Approval Committee respectively, and were certified by Shandong Trionyx sinensis Original Seed Farm in 2006 and National Trionyx sinensis Original Seed Farm in 2007.
In March 2005, Martin Science and Technology Company formulated the "425- 1-30" soft-shelled turtle culture technology, which was appraised by the Provincial Science and Technology Department and was "the first in China". "Research on High-quality Soft-shelled Turtle Breeding Technology and Industrialization Breeding" won the second prize of Shandong Science and Technology Progress Award; In 2005, the high-tech industrialization project of high-quality turtle breeding and green breeding developed by the company was included in the national high-tech industrialization demonstration project; The yellow river strain breeding demonstration project of Trionyx sinensis was listed as a modern agricultural demonstration project by the National Development and Reform Commission. In 2006, "High-tech development of turtle fry breeding" was included in the national spark plan by the Ministry of Science and Technology. Martin Biotechnology Co., Ltd. has made scientific and bold exploration and development in the breeding of soft-shelled turtle, and summed up a set of new techniques and standardized operating procedures for soft-shelled turtle breeding.
Wild parent soft-shelled turtle, the first and second generation "Huang Yun" brand wild parent soft-shelled turtle, and finished soft-shelled turtle are all cultivated by natural ecological biological green technology. The aquaculture water is deep groundwater, and the water quality meets the national aquaculture water quality standards. The reasonable adjustment of zooplankton and zooplankton content with silver carp makes the water quality relatively stable and fresh, which creates a good growth environment for Trionyx sinensis and creates modern breeding water for raising Trionyx sinensis. The feed adopts high-quality full-price feed that meets the national green food standards in a timely and appropriate amount, which provides reliable technical support for the green and healthy breeding of Trionyx sinensis in China. The comprehensive epidemic prevention technology is adopted for the disease prevention of Trionyx sinensis, and the national banned drugs are not used in the breeding of Trionyx sinensis, and limited drugs are used in small quantities or avoided. The specific epidemic prevention technical measures are as follows: cleaning the pond and disinfection; Do a good job of ecological water transfer; Maintain the aquaculture water level; Regular water disinfection; Strictly control feeding; Disinfect tools, personnel and environment on time; Regularly prevent drugs in the body. The disease control of Trionyx sinensis should be timely and directionally controlled in strict accordance with the regulations of NY/T507 1 of the Ministry of Agriculture. Antibiotics with a specified dose should be added to the feed, every month 1 time for 5 days, or Chinese herbal medicine powder should be added to the feed twice a month for 5-7 days, or microecological agents should be often added to the feed to inhibit the reproduction of pathogenic microorganisms in vivo and maintain the ecological balance in vivo. In 200 1 year, the company won the national "Excellent Project Achievement Award" for Chinese herbal medicines.
Since 200 1, the "Huang Yun" brand Trionyx sinensis cultivated by the company with the green technology of natural ecology and biology has been certified by China Green Food Development Center as the first aquatic series "A" grade green food in China; In 2002, it won the "Best-selling Product Award" of China Green Food Fuzhou Expo. In 2005, it was certified by Shandong Green Food Development Center as the top ten brands of green food in Shandong Province; In 2007, it was certified as a famous brand agricultural product in Shandong Province; "Huang Yun" brand trademark is recognized as a famous trademark in Shandong Province, which is deeply trusted and favored by consumers in China.
Years of production practice and technical research have enabled Martin Science and Technology Company to successfully sum up natural temperature control, humidity control and ecological incubation technology, natural temperature control, humidity control and ecological breeding technology of juvenile soft-shelled turtle, and intensive cultivation technology of juvenile soft-shelled turtle at constant temperature, so that the incubation rate of juvenile soft-shelled turtle can reach over 98%. The young soft-shelled turtle cultivated at constant temperature and heat preservation has strong physique and fast weight gain, which is 65438+ higher than that of ordinary soft-shelled turtle cultivated at constant temperature. These breeding techniques have passed the appraisal of Shandong Science and Technology Department and are in a leading position in China.
Martin Biotechnology Co., Ltd. made use of advanced breeding and breeding technology to make the enterprise develop and become the only original seed farm of Trionyx sinensis in China. In 2007, 4.5 million "Huang Yun" brand high-quality Trionyx sinensis fry were bred, and the annual output of "Huang Yun" brand high-quality commodity Trionyx sinensis was 400,000 kg, which drove more farmers to get rich, greatly promoted the development of high-quality Trionyx sinensis breeding industry in China and created higher economic benefits.
Turtle is full of treasures, and its shell, head, meat, blood and gallbladder can all be used as medicine. According to Compendium of Materia Medica, Carapax Trionycis can soften hard mass, disperse hard mass, promote blood circulation and remove blood stasis, nourish yin and replenish blood, and clear away heat and remove blood stasis. Carp can treat chronic dysentery, consumptive tuberculosis and beriberi. Carapax Trionycis is mainly used for bone steaming, fatigue and heat, yin deficiency and wind movement, hepatosplenomegaly and liver cirrhosis. External application of tortoise blood can cure facial paralysis, malnutrition and hot flashes, and has the effect of rapid blood contraction, and mixing wine can cure women's blood consumption; Turtle eggs can cure chronic diarrhea and dysentery; Turtle bile has the effect of treating hemorrhoids and fistula; The dried glans penis is called "glans penis", which can cure proctoptosis and sores. There are as many as 8 kinds of medication sites recorded in the Dictionary of Traditional Chinese Medicine. Turtle is also considered as an effective aphrodisiac. Research by the Department of Pharmacy of the University of Tokyo in Japan believes that eating soft-shelled turtle can quickly eliminate fatigue, and in recent years, athletes are encouraged to take soft-shelled turtle soup before the competition. In 2003, the company cooperated with the General Hospital of the Chinese People's Liberation Army to research and develop turtle gum health food, using domestic advanced multi-stage countercurrent extraction technology to extract turtle gum, using nano-homogeneous spray drying technology to obtain turtle powder, using fluidized bed airflow crushing technology to obtain turtle bone powder, using vacuum drying and supercritical extraction technology to obtain turtle oil, and using advanced edible turtle technology to process turtle convenience food. Through functional research, turtle gum series health food has the functions of anti-fatigue, improving immunity, anti-radiation and strengthening physique. After further research, the turtle glue series health food also has medicinal and health care values such as softening hardness, preventing cancer, lowering blood sugar, softening blood vessels and beautifying. This series of health food is deeply processed by domestic original technology, which greatly improves the effective component content, biological activity and efficacy of the product and fills the gap at home and abroad. The research and development of turtle glue passed the appraisal of scientific and technological achievements in Shandong Province and won the third prize of scientific and technological progress in Shandong Province.