The gel concentration of Biorad protein electrophoresis was 14% to 16%. Biorad protein electrophoresis gel is an anionic detergent, which can denature protein and make each polypeptide chain unfold in a linear direction. Protein or nucleic acid enters the separation gel from the concentrated gel during electrophoresis. Due to the function of molecular sieve, small molecular substances can easily pass through, with small resistance and fast migration speed. Macromolecules are delayed by greater resistance.