1. When using various centrifuges, the centrifuge tube and its contents must be accurately balanced on the balance in advance, and the weight difference during balance shall not exceed the range specified in the instruction manual of each centrifuge. Different rotors of each centrifuge have their own allowable differences, and it is absolutely forbidden to install odd tubes in the rotors. When the rotor is only partially loaded, the pipes must be symmetrically placed in the rotor so that the load can be evenly distributed around the rotor.
2, if you want to centrifuge at a temperature below room temperature. Before use, the rotor should be pre-cooled in the rotor room of refrigerator or centrifuge.
3. Don't leave at will during centrifugation. Observe whether the instruments on the centrifuge work normally at any time. If there is any abnormal sound, stop the machine immediately to check and troubleshoot in time.
4. Every turn has its maximum allowable speed and accumulated time limit. Please refer to the instructions when using the turn, and do not use it too fast. There should be a usage file for each round to record the accumulated usage time. If the maximum use time of turning is exceeded, it must be used at a reduced speed according to the regulations.
5. When loading the solution, it should be carried out according to the specific operating instructions of various centrifuges. According to the nature and volume of the liquid to be centrifuged, an appropriate centrifuge tube should be selected. Some centrifugal tubes have no cover, so they can't contain too much liquid to prevent the head from being thrown out during centrifugation, causing imbalance, rust or corrosion. However, the centrifuge tube of the preparative ultracentrifuge often needs to be filled with liquid to avoid the depression and deformation of the upper part of the plastic centrifuge tube during centrifugation.
After each use, the rotary head must be carefully checked, cleaned and dried in time. Rotating head is the key component of centrifuge. Be careful when moving, don't collide, so as not to cause scars. When the rotary head is not used for a long time, it should be protected by a layer of polishing wax. It is forbidden to use centrifugal tubes that are obviously deformed, damaged or aged.
Extended data:
Rules for use of centrifuges:
1. When the centrifuge is in the pre-cooling state, the centrifuge cover must be closed, and the rotor taken out after centrifugation should be inverted on the experimental platform to dry the residual water in the cavity. At this point, the centrifuge cover is open.
2. When the rotor is precooled, the rotor cover can be placed on the centrifuge platform or experimental platform. Never tighten it and float on the rotor, because once it is started by mistake, the rotor cover will fly out, causing an accident!
3. After tightening the rotating cover, be sure to touch the gap between the rotating cover and the rotating cover with your fingers. If there is a gap, unscrew and re-tighten it until it is confirmed that there is no gap before starting the centrifuge.
4, in the process of centrifugation, the operator shall not leave the centrifuge room, in case of abnormal situation, the operator can't turn off the power (power), to press the stop button. Fill in the centrifuge usage record before precooling.
5. Fake centrifugal tubes, aged, deformed and cracked centrifugal tubes shall not be used.
6. The last person who uses centrifuges on holidays and nights can only leave after routine safety inspection.
7. During the use of the instrument, please contact the manufacturer in time in case of machine failure and component damage.
Precautions for using centrifuges:
1, the bottom of centrifuge shell should be padded with cotton or test tube pad.
2, electric centrifuge, if the fuselage has noise or vibration, should immediately cut off the power supply, troubleshooting immediately.
3. Centrifugal tubes must be placed symmetrically in the casing to prevent the vibration of the fuselage. If there is only one sample quality control, the other should be replaced with water of the same quality.
4. When starting the centrifuge, cover the top cover of the centrifuge before starting it slowly.
5. After separation, turn off the centrifuge first. After the centrifuge stops rotating, you can open the centrifuge cover and take out the sample. No external force can force it to stop moving.
6. The centrifugation time is generally 65438 0 ~ 2 minutes, during which the experimenter is not allowed to leave to do other things.
Reference source: Baidu Encyclopedia-Centrifuge