High-throughput sequencing, also known as the "next generation" sequencing technology, is characterized by being able to measure hundreds of thousands to millions of DNA molecules in parallel at one time, and generally has a short reading length.

Noun interpretation

According to the different development history, influence, sequencing principle and technology, there are mainly the following kinds: massive parallel signature sequencing, MPSS, Polony sequencing, 454 pyrophosphate sequencing, Illumina (Solexa) sequencing, ABI solid-state sequencing, ionic semiconductor sequencing, DNA nanosphere sequencing and so on.

High-throughput sequencing technology is a revolutionary change to traditional sequencing, which can sequence hundreds of thousands to millions of DNA molecules at a time, so it is called the next generation sequencing in some literatures, which shows its epoch-making change. At the same time, high-throughput sequencing makes it possible to analyze the transcriptome and genome of a species in detail, so it is also called deep sequencing.

meaning

The birth of high-throughput sequencing technology can be said to be a milestone in the field of genomics research. This technology greatly reduces the single base cost of nucleic acid sequencing compared with the first generation sequencing technology. Taking human genome sequencing as an example, the Human Genome Project launched at the end of last century spent $3 billion to decode human life codes, and the second-generation sequencing made human genome sequencing enter the era of 10,000 yuan (US) genome. Such low-cost single-base sequencing enables us to implement genome projects of more species to decrypt the genome genetic codes of more biological species. At the same time, in the species that have completed genome sequencing, it is also possible to re-sequence the whole genome of other varieties of this species on a large scale. [3]?