DNA fragments with genetic information are called genes and other DNA sequences, some of which directly work with their own structures, while others participate in regulating the expression of genetic information. It takes at least 265 to 350 genes to make up a simple life. (This involves the power of the gene working group. The human gene working group is basically similar to the fruit fly.)
Question 2: What is common gene sequencing, and what is the difference between it and high-throughput sequencing? Because of your answer, according to the different development history, influence, sequencing principle and technology, there are mainly the following: massive parallel signature sequencing, MPSS, Polony sequencing, 454 pyrophosphate sequencing, Illumina (Solexa) sequencing, ABI solid-state sequencing, ionic semiconductor sequencing, DNA nanosphere sequencing and so on.
High-throughput sequencing technology is a revolutionary change to traditional sequencing, which can sequence hundreds of thousands to millions of DNA molecules at a time, so it is called the next generation sequencing in some literatures, which shows its epoch-making change. At the same time, high-throughput sequencing makes it possible to analyze the transcriptome and genome of a species in detail, so it is also called deep sequencing.
Question 3: What is general gene sequencing? Is it different from high-throughput sequencing? "General gene sequencing" should refer to "conventional DNA sequencing", that is, a method of Sanger method (namely dideoxy method) sequencing. At present, it is very common to use ABI 3730xl directly for automatic sequencing, and the reading length can basically be 600bp-800bp.
High-throughput sequencing's concept is actually a relative concept. In 2000, sequencing on 3700, MegaBace and other instruments was also a high-throughput sequencing, which was relative to manual sequencing or running gel.
However, after 2005, high-throughput sequencing changed to refer to the second generation sequencing, such as 454, Solexa (later changed to Illumina) and SOLiD, which increased the throughput by thousands or even hundreds of millions of times compared with the first generation sequencing such as 3730, so it was called high-throughput sequencing.
The characteristics of NGS mainly include:
1, Qualcomm quantity. One run can generate 500Mb to 600Gb of data.
2. The reading length is relatively short. 454 (about 400-500bp), llumina( 100-250bp), solid (75- 100).
3. The unit data cost is very low. The cost of sequencing many projects now. It's already low. The cost of bioinformatics analysis becomes more important.
Question 4: What does the gene sequencing board mean? Gene sequencing package, the context should be introduced, or is there anything specific to understand?