When a cell line is established, China often organizes experts to hold identification meetings to identify it. From an academic perspective, this is not necessary. According to international practice, as long as the research is carried out seriously and responsibly, the relevant information is reported in magazines or publications and the above-mentioned items are introduced in detail.

In the past, when a unified cell bank was not established, the established cell lines (strains) were mostly kept by the author's institution, which resulted in waste of manpower, inconvenience of communication and use, and easy contamination and loss of cells. Our country has initially established a small-scale cell storage institution, and further development will surely have a greater role in promoting cell culture in our country. When ATCC accepts banked cells, they must meet its storage standards. The test items required for ATCC banked cells are as follows:

Cultivation resume: tissue source date, species, tissue origin, gender, age, donor is normal or Abnormal health status, number of cell passages, etc.

Cryopreservation solution: name of culture medium and antifreeze solution

Cell viability: survival rate and growth characteristics of cell inoculation before and after thawing

Culture medium: culture medium type and name (generally required not to contain antibiotics), serum source and content

Cell morphology: type, such as epithelial or fibroblasts, cell growth characteristics after thawing

Karyotype: diploid or polyploid, presence or absence of marker chromosomes

Contamination-free detection: including bacteria, fungi, mycoplasma, protozoa and viruses, etc.

Species detection: detection of isoenzymes, mainly G6PD and LDH, to prove whether cells have cross-contamination and reverse transcriptase detection

Immuno detection: one or two serological tests

Cell founder: founder’s name; detector’s name