2. Grafting. Grafting can be done from September to 65438+10, and the survival rate is high. First of all, when taking buds, choose the annual strong branches growing at the lower part of peony, which are 6- 10 cm long, with stout terminal buds and 2-3 lateral buds. When picking buds, use them as they are and protect them when not in use. After selecting the grafted bud, obliquely cut a knife on the back of the bud at the lower part of the grafted bud to form a horse ear shape, and then obliquely cut the other side of the horse ear shape into a wedge shape, so that after grafting, both sides can meet the included angle between xylem and phloem to form a tissue, which is easier to survive.
3. Cutting propagation. Cutting propagation is a method of propagating new plants by using adventitious roots of peony branches. First, the cuttings are cut and separated from the mother plant, and then inserted into soil or other substrates to make them take root and become new plants. The cuttings should be the tender branches on the roots of the year. When cutting, pour one edge after cutting and pour it thoroughly at one time.
4. sow and breed. Seed propagation is to breed offspring or select new varieties from seeds. Disinfect the soil before sowing, and the soil should be deeply ploughed and fully fertilized. Then build a small border 70 ~ 80 cm wide. Don't sow too deep, take 3 ~ 4 cm as the degree. After sowing, the soil should be flush with the main surface. Then gently stabilize the soil and water it.
5. The sense of hierarchy reappears. Layering propagation is a method of propagation based on the principle that branches can produce adventitious roots. First, the branches are crushed or buried with soil to keep the soil moist, and the technical branches take root in the buried place, and then they are planted by cutting to become new plants.
6. Tissue culture. Tissue culture is based on the totipotency of plant tissue cells, and peony embryos, buds, stem tips, tender leaves and petioles are used for in vitro culture. Soak these materials in 75% essence for 5- 10 minute, immediately put them into sterile water for cleaning, then soak them in 5% antifungal agent solution for 7- 10 minute for surface sterilization, then rinse them with sterile water for 3-4 times, and finally put them on the culture medium for sterile culture.