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Windows Defender in Windows 8.1 Update 1 cannot be started, and a digital signature error message appears when opening the service. What should I do?

This is normal. According to your screenshot, you have installed 360 anti-virus software. Starting from Windows 8, as long as you install anti-virus software, Windows Defender will automatically turn off. Even if you start it manually, something will go wrong. Just leave it alone. Anti-virus software cannot coexist with the system's own software in Windows 8 and 8.1. So it will automatically close.