(instrumental method)
1 experimental principle: sheath flow DC detection method (red blood cells, platelets, HCT)
P-LCR: that is, the large platelet ratio above the recognition value 12fL refers to the ratio of fixed recognition value to particles between LD and UD.
After analyzing the number of platelets, calculate the proportion of large platelets, and the formula is as follows: P-LCR= number of large platelets /PLT× 100%.
2 specimen collection
2. 1 venous blood anticoagulated with EDTA-K2 was collected by vacuum blood collection tube. Immediately after blood collection, mix with anticoagulant, and paste the document number on the outer wall of the test tube.
Personal information or serial number shall be inspected at room temperature immediately after inspection. When venous blood cannot be collected, capillary blood can be collected.
2.2 puncture should prevent the blood sampler from being punctured, so as to avoid carrying infectious pathogens such as hepatitis virus and human immunodeficiency virus in the blood.
Unknown pathogenic components in blood pollute the blood sampler.
3 specimen storage
Specimens should be stored at room temperature for up to 4 hours. Specimens that cannot be detected within 4 hours should be kept in a refrigerator (2℃~8℃) and be valid within 8 hours. Before use, they should be taken out of the refrigerator and put back to room temperature for testing. .
4 specimen transportation: low temperature transportation.
5. Rejection criteria of samples: anticoagulant does not meet the requirements, blood collection is inaccurate, and samples such as coagulation cannot be determined.
6. Reagent:
6. 1. Diluent (battery pack)
6. 1. 1 reagent brand: Sysmex code: PK-30L packing specification: 20L 6. 1.2 reagent composition:
Sodium chloride 6.38g/L boric acid1.00g/L sodium tetraborate 0.20g/L EDTA-K2.
0.20 g/l
6. 1.3 storage conditions: it must be stored in a clean environment to prevent direct sunlight, and the suitable temperature is 15~30℃. 6. 1.4 service life: used before the expiration date; The service life after opening is 60 days.
6. 1.5 safety: confirm whether there is pollution or discoloration before use. Once found, please replace it immediately. If there is any freezing phenomenon, it should be thawed and stirred evenly before use. 6. 1.6 Note: Pay attention to dust prevention.
6.2 Leukolysin (stromatolite -4DL)
6.2. 1 reagent brand: Sysmex code: FFD-200A packing specification: 5L 6.2.2 reagent composition:
Nonionic surfactant 0. 18% organic quaternary ammonium salt 0.08%
6.2.3 Storage conditions: It must be stored in a clean environment, and the suitable temperature is 5~30℃. 6.2.4 Service life: used before the expiry date; The service life after opening is 60 days.
6.2.5 Safety: Avoid contact with eyes and skin. Once in contact, rinse with plenty of water and take appropriate medical measures. 6.2.6 Note: Before using the reagent, it is necessary to confirm whether there is pollution or discoloration. Once found, it must be replaced quickly. If the reagent is frozen, thaw it before use and mix it thoroughly before use. 6.3 leucocyte classification dye (stromatolite -4DS)
6.3. 1 reagent brand: Sysmex code: FFS-800A packing specification: 42ml×3 bags 6.3.2 reagent composition:
Polymethylene dye 0.002% methanol 3.0% 1.2- glycol 96.9%
6.3.3 Storage conditions: It must be stored in a clean environment, away from light, and the suitable temperature is 2~35℃. 6.3.4 Service life: used before the expiry date; The service life after opening is 60 days.
6.3.5 Safety: Avoid contact with eyes and skin. Once in contact, rinse with plenty of water and take appropriate medical measures. 6.3.6 Note: If the reagent shows any signs of pollution, turbidity, instability or color change, it must be replaced. Avoid freezing. 6.4 cleaning agent (CELLCLEAN)
6.4. 1 reagent brand: Sysmex code: CL-50 packing specification: 50ml 6.4.2 reagent composition: sodium hypochlorite (available chlorine concentration is 5%).
6.4.3 Storage conditions: It must be stored in a clean environment to prevent direct sunlight, and the suitable temperature is 1~30℃. 6.4.4 Service life: used before the expiration date.
6.4.5 Safety: This product is a strongly acidic detergent. Avoid contact with eyes, skin and clothes. Once in contact, rinse with plenty of water and take appropriate medical measures.
6.4.6 Note: If the reagent shows any signs of pollution, turbidity, instability or color change, it must be replaced. If the reagent solidifies, it must be replaced.
6.5 Use of reagents:
6.5. 1 All reagents above shall be marked with the opening date and expiration date (2 months after opening the bottle or within the expiration date) and signed.
7. Instruments and equipment:
7. 1 instrument name: Sysmexxs- all automated hematology analyzer 7.2 instrument manufacturer: Japanese Symex company 7.3 instrument model: Symexxs-
7.4 Instrument calibration procedure (see XS calibration instructions-operating instructions for details):
8. Operating steps:
8. 1 Do quality control after startup, and conduct sample testing after quality control passes. 8. 1. 1 Perform sample analysis in rack mode. 8. 1.2 Make sure the instrument is ready. The ready indicator should light up. 8. 1.3 Double-click the "Controller" icon on the menu screen. Displays the controller menu.
8. 1.4
Double-click the syringe sample number icon on the controller menu, or click the syringe button on the toolbar. The sampler sample number dialog box pops up.
8. 1.5 Enter the specimen ID number, or automatically read the ID number through barcode scanning.
8. 1.6 Check the number of the first pipe rack and the position number of the specimen tube. If you need to change, click the item you want to change and enter a value. 8. 1.7 Check the on-demand settings. If you need to change the settings, click the corresponding Discrete to set them.
8. 1.8
Put the sample tube into the sample rack, and then put the sample rack into the sample rack slot on the right side of the injector. Up to 5 sample racks can be loaded at a time.
8. 1.9
After placing the rack in the injector, click Start Injector in the injector sample number dialog box.
8. 1. 10 Check the rack number and tube position number in the rack number/tube position confirmation dialog box. Then click OK 8. 1. 1 1. When all sample tubes are moved to the rack slot on the left side of the injector, the ready LED lights up.
8. 1. 12 specimen detection: In order to verify the manual and automatic modes, two new blood samples were randomly taken every day, and the results of closed and open modes were compared.
Yes, take close as the target value (close-open)/the offset (CV%) of close to meet the comparison requirements, and test the patient samples after the above tests pass.
8. 1. 13 Pay attention to personal safety during operation. If you need to open the specimen, please wear a protective mirror cover or open it behind the plexiglass baffle. 8.2 Daily maintenance: (Maintenance after daily measurement and before shutdown. )
8.2. 1 After the analysis, the word "Ready" will be displayed on the screen. When the green light of the ready LED is on, press the [Shutdown] key. 8.2.2 Place CELLCLEAN on the manual pipetting needle and press the start switch. 8.2.3 When the ready LED light goes out and the buzzer stops, remove the battery cleaner.
9. Judgment and analysis of test results:
9. 1 P-LCR reference range:
Serial number parameter male and female unit
1 P-LCR
9.2 When the platelet count, distribution or morphology is abnormal, the calculation parameters cannot be accurately obtained, and further microscopic examination is needed for confirmation.
10. Quality control:
10. 1 Do quality control every day after startup and compare the results of two injection modes, and then conduct sample analysis and report the results during quality control. 10.2 quality control must be determined in quality control mode, not in sample mode. (See indoor blood quality for details of quality control procedures.
Control work instruction. ) 10.3 storage conditions: 2℃ ~ 8℃
10.4 service life: Sysmex e-CHECK(XS) 14 days after the quality control is opened. 10.5 After the quality control is started, the start date, expiration date and signature shall be indicated.
10.6 quality control frequency: analyze the quality control products once a day after startup and before specimen testing.